Melt 50 ml of 2XTA and allow it to cool to ~55°C. Using aseptic technique, add the CaCl 2 and 7H9 broth to the melted agar. Aseptically dispense ml of the mixture per 13 mm tube and store in a 55°C incubator ≤7 days. Cooling MBTA to room temperature or 4°C will cause the CaCl 2 .
Delmass – Fortified Cave. Mgharet Delmass, loed in the village of Beit Munzer on the outskirts of the Qadisha Valley was explored by the GERSL in 1995 and published in 1998 (Liban Souterrain 5.). This fortified cave dated back to the 13th 14th century overlooked the strategic path linking the coastal city of Tripoli to Baalbeck and ...
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